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circRNA Overexpression Vector

The upgraded version of circRNA overexpression vector (plasmid, lentivirus), the overexpression efficiency is as high as 1000 times! The cyclization efficiency is as high as 95%!
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PRODUCT INFORMATION

Product introduction:
circRNA is a type of endogenous ncRNA molecule that exists widely and diversely in a variety of biological cells and has the function of regulating gene expression.In the current research, it is only clear that circRNA has the sponge effect of adsorbing miRNA and regulating gene transcription.However, in order to further clarify the formation mechanism and function of circRNA in organisms, more circRNA related data are needed to support it.Therefore, circRNA overexpression vectors play an indispensable instrumental role in the study of the functional mechanism of circRNA.
Using patented technology, BersinbioTM has successfully developed an expression vector that specifically and accurately overexpresses circRNAs, and is the first to provide researchers with a simple and efficient circRNAs overexpression research strategy. Two kinds of circRNAs expression vectors have been launched, which are suitable for common eukaryotic expression, lentivirus packaging, green fluorescence and puromycin screening. The vector contains the circRNAs circular expression framework of BersinbioTM 's patented technology. After the vector is transfected into cells, the circRNAs molecules can be formed by RNA splicing. Researchers only need to use PCR to amplify the linear sequence of the target circRNAs and clone it into an expression vector, which greatly simplifies the experimental steps and saves time. (For scientific research purposes only)





Technical process:








The upgraded version of circRNA overexpression vector is here!






Product features:
* The upgraded version of circRNA overexpression vector (plasmid, lentivirus), the overexpression efficiency is as high as 1000 times! The cyclization efficiency is as high as 95%!
1、High overexpression efficiency——circRNAs expression vector is equipped with the upstream and downstream cyclization framework of BersinbioTM 's patented technology, and all circRNAs verified can be significantly overexpressed.
2、Accurate sequence circularization——The circularization-mediated sequence of BersinbioTM 's patented technology is introduced into the circularization framework, which can effectively ensure the accurate circularization of the linear sequence of the target circRNAs. All circRNAs verified can be accurately circularized without base addition or deletion.
3、High stability of overexpression——The verified target circRNAs can achieve accurate and efficient overexpression with sequences ranging from 200bp to 2500bp.
4、Wide range of applications——suitable for common eukaryotic expression, lentivirus packaging, green fluorescence and puromycin screening and other purposes.


Article


【In 2023


ArticleExosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis

PeriodicalsCancer Cell International

IF:5.800


【In 2021


ArticleCircular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

PeriodicalsCell Death & Disease

IF8.469


ArticleCircular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis

PeriodicalsCell Biology and Toxicology

IF6.69


ArticleCircRNA_0044556 diminishes the sensitivity of triplenegative breast cancer cells to adriamycin by sponging miR145 and regulating NRAS

PeriodicalsMolecular Medicine REPORTS

IF2.95


ArticleCircRNA hsa_circ_0005909 Promotes Cell Proliferation of Osteosarcoma Cells by Targeting miR-338-3p/HMGA1 Axis

PeriodicalsCancer Management and Research

IF2.886


ArticleCircular RNA CircCDYL Regulates Proliferation and Apoptosis in Non-Small Cell Lung Cancer Cells by Sponging miR-185-5p and Upregulating TNRC6A

PeriodicalsCancer Management and Research

IF2.886


【In 2020


ArticleCircECE1 activates energy metabolism in osteosarcoma by stabilizing c-Myc

PeriodicalsMolecular Cancer

IF15.302


ArticleCirc_2858 helps blood-brain barrier disruption by increasing VEGFA via sponging miR-93-5p during Escherichia coli meningitis

PeriodicalsMolecular Therapy: Nucleic Acid

IF7.032


ArticleSuper-Enhancer LncRNA LINC00162 Promotes Progression of Bladder Cancer

PeriodicalsiScience

IF4.447


ArticleCirc_0084927 Facilitates Cervical Cancer Development via Sponging miR-142-3p and Upregulating ARL2

PeriodicalsCancer Management and Research

IF2.886


ArticleCircular RNA Circ-ZNF609 Promotes Lung Adenocarcinoma Proliferation by Modulating miR-1224-3p/ETV1 Signaling

PeriodicalsCancer Management and Research

IF2.886


【In 2019


ArticleCircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene

PeriodicalsAnnals of the Rheumatic Diseases

IF14.299


ArticleCircLMNB1 promotes colorectal cancer by regulating cell proliferation, apoptosis and  epithelial-mesenchymal transition

PeriodicalsOncoTargets and Therapy

IF3.337


Article:CircLMTK2 acts as a novel tumor suppressor in gastric cancer

Periodicals:Bioscience Reports

IF:2.942


【In 2018


ArticleCircular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

PeriodicalsAdvanced Science

IF17.521


ArticleCircular RNA transcriptomics analysis of primary human brain microvascular endothelial cells infected with meningitic Escherichia coli

PeriodicalsMolecular Therapy: Nucleic Acid

IF7.032


【In 2017


Article:TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway

Periodicals:Journal of Hematology & Oncology

IF:17.388