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N4-acetylcytidine (ac4C) is a highly conserved RNA acetylation modification that is widely present in tRNA, rRNA, and mRNA of both eukaryotes and prokaryotes. It is significantly associated with a variety of human diseas......
RNA methylation refers to the methylation modification phenomenon that occurs at different positions on the RNA molecule. The common methods of RNA post-transcriptional modification include m6A, m5C, m7G and m1A....
RNA methylation refers to the methylation modification phenomenon that occurs at different positions on the RNA molecule. The common methods of RNA post-transcriptional modification include m6A, m5C, m7G and m1A....
RNA methylation refers to the methylation modification phenomenon that occurs at different positions on the RNA molecule. The common methods of RNA post-transcriptional modification include m6A, m5C, m7G and m1A....
A specific probe is designed for the target area and labeled with biotin.The end-labeled nucleic acid probe can bind to the protein. During electrophoresis, the probe and the protein complex move slowly in the gel, that ......
A specific probe is designed for the target area and labeled with biotin.The end-labeled nucleic acid probe can bind to the protein. During electrophoresis, the probe and the protein complex move slowly in the gel, that ......
RNA methylation refers to the methylation modification phenomenon that occurs at different positions on the RNA molecule. The common methods of RNA post-transcriptional modification include m6A and m5C....
MeDIP enriches highly methylated DNA fragments with 5mC antibody, and uses downstream nucleic acid detection technology to detect methylation sites in the whole genome....
Co-IP is a classic method for studying protein interactions based on the specificity of binding between antibodies and antigens....
The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system....
CLIP-seq obtains purified and digested (Protease K, DNase I, RNase T1) RNA by immunoprecipitating the binding complex of RBP and RNA, and performs cDNA library construction and sequencing analysis....
Red blood cell lysate uses the principle of rupture of the cell membrane caused by the difference in the concentration of salt ions inside and outside the cell to lyse the non-nucleated red blood cells....
Northern blot is a method of transferring RNA from a gel to a nylon membrane, and detecting gene expression by detecting the level of RNA expression....
CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation....