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Co-IP is a classic method for studying protein interactions based on the specificity of binding between antibodies and antigens....
The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system....
CLIP-seq obtains purified and digested (Protease K, DNase I, RNase T1) RNA by immunoprecipitating the binding complex of RBP and RNA, and performs cDNA library construction and sequencing analysis....
Red blood cell lysate uses the principle of rupture of the cell membrane caused by the difference in the concentration of salt ions inside and outside the cell to lyse the non-nucleated red blood cells....
Northern blot is a method of transferring RNA from a gel to a nylon membrane, and detecting gene expression by detecting the level of RNA expression....
CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation....