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DNA-EMSA Kit

A specific probe is designed for the target area and labeled with biotin.The end-labeled nucleic acid probe can bind to the protein. During electrophoresis, the probe and the protein complex move slowly in the gel, that is, it shows a relative lag.
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PRODUCT INFORMATION

Product introduction：
EMSA is a classic method for studying promoter binding proteins and a technique for qualitative and quantitative analysis of nucleic acid-protein interactions.This method is not only simple, rapid and highly sensitive; on the other hand, it can be used to evaluate the properties of protein and nucleic acid binding by using a competition test.It is currently used to detect DNA-binding proteins, and can be used to detect specific proteins by adding specific antibodies. The binding protein two-dimensional electrophoresis and mass spectrometry techniques can be used for identification and analysis of unknown proteins.(This kit is for scientific research purposes only)
A specific probe is designed for the target area and labeled with biotin.The end-labeled nucleic acid probe can bind to the protein. During electrophoresis, the probe and the protein complex move slowly in the gel, that is, it shows a relative lag.






Technical process：







Product features：

1.Safety and environmental protection: The non-isotopic chemiluminescence detection system is adopted to avoid the danger of radioactivity and the trouble of handling isotope waste, and reduce environmental pollution; no special isotope operation room is required, and it is easy to use and promote.
2.High sensitivity: The detection sensitivity is high, which is equal to or exceeds the isotope labeling system, and the detection result background is lower.
3.Fast and time-saving: From labeling probes to result analysis, all experiments only take 5 hours.
4.Stable and reliable: The RNA ends are labeled with biotin, which does not affect the protein binding site; the labeled RNA can be stored for one year.
5.Equipped with complete equipment: equipped with BersinbioTM cell nucleus extraction kit.

6.Personalized service: personalized high-sensitivity probes can be customized according to research needs.


Instructions


Bes5003 DNA-EMSA  

Bes5107 RNA-EMSA 


Articles


【In 2023】


Article：The RNA-binding protein hnRNP F is required for the germinal center B cell response

Periodicals：Nature Communications

IF：16.600


Article：An inducible long noncoding RNA, LncZFHX2, facilitates DNA repair to mediate osteoarthritis pathology

Periodicals：Redox Biology

IF：11.4


Article：OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers

Periodicals：Rice

IF：5.500


【In 2022】


Article：ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis

Periodicals：International Journal of Biological Sciences

IF：9.200


Article：GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus

Periodicals：International Journal of Molecular Sciences

IF：5.600


【In 2021】


Article：Global identification of full‐length cassava lncRNAs unveils the role of cold‐responsive intergenic lncRNA 1 in cold stress response

Periodicals：Plant Cell Environ

IF：7.300


Article：VPS33B suppresses lung adenocarcinoma metastasis and chemoresistance to cisplatin

Periodicals：Genes & Diseases

IF：7.103


Article：miR-1254 induced by NESG1 inactivates HDGF/DDX5-stimulated nuclear translocation of b-catenin and suppresses NPC metastasis

Periodicals：Molecular Therapy: Methods & Clinical Development

IF：6.698


Article：An insertion variant of MGMT disrupts a STAT1 binding site and  confers susceptibility to glioma

Periodicals：Cancer Cell International

IF：5.722


Article：A direct negative feedback loop of miR-4721/FOXA1/Nanog promotes nasopharyngeal cell stem cell enrichment and  metastasis

Periodicals：Journal of Translational Medicine

IF：5.531


Article：VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer

Periodicals：Cancer Science

IF：4.965


【In 2020】


Article：Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma

Periodicals：Signal Transduction and Targeted Therapy

IF：13.493


Article：MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.889


Article：circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.889


Article：miR-4721, Induced by EBV-miR-BART22, Targets GSK3b to Enhance the Tumorigenic Capacity of NPC through the WNT/b-catenin Pathway

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.886


Article：MicroRNA-4476 promotes glioma progression through a miR-4476/APC/β-catenin/c-Jun positive feedback loop

Periodicals：Cell Death & Disease

IF：8.469


Article：MiR-4310 induced by SP1 targets PTEN to promote glioma progression

Periodicals：Cancer Cell International

IF：4.178


【In 2019】


Article：Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression

Periodicals：NATURE COMMUNICATIONS

IF：12.123


Article：Timeless-Stimulated miR-5188-FOXO1/b-Cateninc-Jun Feedback Loop Promotes Stemness via Ubiquitination of b-Catenin in Breast Cancer

Periodicals：Molecular Therapy

IF：11.455


Article：HBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma

Periodicals：Theranostics

IF：8.698


Article：VPS33B interacts with NESG1 to modulate EGFR/PI3K/AKT/c-Myc/P53/miR-133a-3p signaling and induce 5-fluorouracil sensitivity in nasopharyngeal carcinoma

Periodicals：Cell Death & Disease

IF：8.469


Article：Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway

Periodicals：EBioMedicine

IF：5.736


Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant

Periodicals：Cellular and Molecular Neurobiology

IF：3.606


【In 2016】


Article：miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR–p-PI3K/AKT-c-JUN

Periodicals：NATURE COMMUNICATIONS

IF：14.92