RAP Kit

Product introduction：
RAP is used in experiments to study the interaction between RNA and DNA, RNA and protein.According to different research objects, RAP technology combines high-throughput sequencing (RAP-Seq) and mass spectrometry (RAP-MS) to study the genes and proteins that interact with target RNA.(This kit is for scientific research purposes only)
RAP technology is to design a biotin or streptavidin probe set. After the target RNA is pulled down, the DNA, RNA or protein (RBPs) that interact with it will be attached to the magnetic beads.Finally, perform high-throughput sequencing of the gene, so that the downstream target gene that regulates RNA transcription regulation will be obtained.At the same time, RBPs can carry out Western Blot verification or MS identification of unknown proteins.
BersinbioTM RAP Kits include: RAP-RNA Kit, RAP-Protein Kit and RAP-RNA, Protein Kit.






Technical process：







Product features：
1.The test has high sensitivity and good stability.
2.Leading detection methods, accurate results and good repeatability.

3.BersinbioTM is exclusively developed and has independent intellectual property rights.

Instructions

Bes5103-1 RAP-RNA

Bes5103-2 RAP-Protein

Bes5103-3 RAP-RNA Protein

Articles

【In 2023】

Article：Circular RNA circ0001955 promotes cervical cancer tumorigenesis and metastasis via the miR-188-3p/NCAPG2 axis

Periodicals：Journal of Translational Medicine

IF：7.400

Article： N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes

Periodicals：iScience

IF：5.800

Article：circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization

Periodicals：iScience

IF：5.800

Article：hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffness.

Periodicals：Aging (Albany NY)

IF：5.200

Article：lhCLIP reveals the in vivo RNA-RNA interactions recognized by hnRNPK

Periodicals：PloS Genetics

IF：4.500

Article：LincRNA00612 inhibits apoptosis and  inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter

Periodicals：PeerJ

IF：2.700

【In 2022】

Article：The N6-methyladenosine modifcation of circALG1 promotes the metastasis of colorectal cancer mediated by the miR-342-5p/PGF signalling pathway

Periodicals：Molecular Cancer

IF：37.300

Article：EIF4A3-induced circTOLLIP promotes the progression of hepatocellular carcinoma via the miR-516a-5p/PBX3/EMT pathway

Periodicals：Journal of Experimental & Clinical Cancer Research

IF：11.300

Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

Periodicals：Cell Death and Disease

IF：9.000

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death and Disease

IF：8.469

Article：The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by bindingmRNA and regulating its stability

Periodicals：Molecular Therapy: Oncolytics

IF：6.311

Article：Long Non‑coding Antisense RNA DDIT4‑AS1 Regulates Meningitic Escherichia coli‑Induced Neuroinfammation by Promoting DDIT4 mRNA Stability

Periodicals：Molecular Neurobiology

IF：5.59

Article：Long non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

Periodicals：Molecular Brain

IF：4.04

【In 2021】

Article：LINC00473-modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid-induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

Periodicals：Bioengineering & Translational Medicine

IF：7.400

Article：Circular RNA hsa_circ_0043280 inhibits cervical cancer tumor growth and metastasis via miR-203a-3p/PAQR3 axis

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circACSL1 aggravated myocardial inflammation and myocardial injury by sponging miR-8055 and regulating MAPK14 expression

Periodicals：Cell Death & Disease

IF：8.469

Article：CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition

Periodicals：Cell Death & Disease

IF：8.469

Article：Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

Periodicals：Cell Death & Disease

IF：8.469

Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity

Periodicals：Cell Death & Disease

IF：8.469

Article：lncRNA GAS5, as a ceRNA, inhibits the proliferation of diffuse large B‑cell lymphoma cells by regulating the miR‑18a‑5p/RUNX1 axis

Periodicals：INTERNATIONAL JOURNAL OF ONCOLOGY

IF：5.65

Article：CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5

Periodicals：Frontiers in Molecular Neuroscience

IF：5.630

Article：uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation

Periodicals：Frontiers in Oncology

IF：4.848

Article：CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and  inhibit glioma progression

Periodicals：Cancer Cell International

IF：4.175

Article：lncRNA RP11‑531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression

Periodicals：Experimental and Therapeutic Medicine

IF：2.447

【In 2020】

Article：circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p

Periodicals：Molecular Therapy: Nucleic Acids

IF：8.800

Article：CircRNA104250 and lncRNAuc001.dgp.1 promote the PM2.5-induced inflammatory response by co-targeting miR-3607-5p in BEAS-2B cells

Periodicals：Environmental Pollution

IF：6.792

Article：The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis

Periodicals：Cell Death & Disease

IF：6.302

【In 2019】

Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

Periodicals：Advanced science

IF：15.100

Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Periodicals：Molecular Cancer

IF：7.776

Article：CircPCMTD1 Acts as the Sponge of miR-224-5p to Promote Glioma Progression

Periodicals：Frontiers in Oncology

IF：4.137

Article：Mechanism of aberrant long non-coding RNA expression in an adriamycin-resistant liver cancer cell strain

Periodicals：Digestive and Liver Disease

IF：4.088

【In 2016】

Article：A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis

Periodicals：Archives of Toxicology

IF：5.728