Product introduction：
EMSA is a classic method for studying specific nucleic acid binding proteins, and is a technique for qualitative and quantitative analysis of nucleic acid-protein interactions.This method is not only simple, rapid and highly sensitive; on the other hand, it can be used to evaluate the properties of protein and nucleic acid binding by using a competition test.It is currently used to detect RNA-binding proteins, and can be used to detect specific proteins by adding specific antibodies. The binding protein two-dimensional electrophoresis and mass spectrometry techniques can be used for identification and analysis of unknown proteins.(This kit is for scientific research purposes only)
A specific probe is designed for the target area and labeled with biotin.The end-labeled nucleic acid probe can bind to the protein. During electrophoresis, the probe and the protein complex move slowly in the gel, that is, it shows a relative lag.
Technical process：
Product features：
1.Safety and environmental protection: The non-isotopic chemiluminescence detection system is adopted to avoid the danger of radioactivity and the trouble of handling isotope waste, and reduce environmental pollution; no special isotope operation room is required, and it is easy to use and promote.
2.High sensitivity: The detection sensitivity is high, which is equal to or exceeds the isotope labeling system, and the detection result background is lower.
3.Fast and time-saving: From labeling probes to result analysis, all experiments only take 5 hours.
4.Stable and reliable: The RNA ends are labeled with biotin, which does not affect the protein binding site; the labeled RNA can be stored for one year.
5.Equipped with complete equipment: equipped with BersinbioTM cell nucleus extraction kit.
6.Personalized service: personalized high-sensitivity probes can be customized according to research needs.
Instructions
Bes5003 DNA-EMSA
Bes5107 RNA-EMSA
Articles
【In 2023】
Article：The RNA-binding protein hnRNP F is required for the germinal center B cell response
Periodicals：Nature Communications
IF：16.600
Article：An inducible long noncoding RNA, LncZFHX2, facilitates DNA repair to mediate osteoarthritis pathology
Periodicals：Redox Biology
IF：11.4
Article：OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers
Periodicals：Rice
IF：5.500
【In 2022】
Article：ENKUR expression induced by chemically synthesized cinobufotalin suppresses malignant activities of hepatocellular carcinoma by modulating β-catenin/c-Jun/MYH9/USP7/c-Myc axis
Periodicals：International Journal of Biological Sciences
IF：9.200
Article：GcvB Regulon Revealed by Transcriptomic and Proteomic Analysis in Vibrio alginolyticus
Periodicals：International Journal of Molecular Sciences
IF：5.600
【In 2021】
Article：Global identification of full‐length cassava lncRNAs unveils the role of cold‐responsive intergenic lncRNA 1 in cold stress response
Periodicals：Plant Cell Environ
IF：7.300
Article：VPS33B suppresses lung adenocarcinoma metastasis and chemoresistance to cisplatin
Periodicals：Genes & Diseases
IF：7.103
Article：miR-1254 induced by NESG1 inactivates HDGF/DDX5-stimulated nuclear translocation of b-catenin and suppresses NPC metastasis
Periodicals：Molecular Therapy: Methods & Clinical Development
IF：6.698
Article：An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma
Periodicals：Cancer Cell International
IF：5.722
Article：A direct negative feedback loop of miR-4721/FOXA1/Nanog promotes nasopharyngeal cell stem cell enrichment and metastasis
Periodicals：Journal of Translational Medicine
IF：5.531
Article：VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer
Periodicals：Cancer Science
IF：4.965
【In 2020】
Article：Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma
Periodicals：Signal Transduction and Targeted Therapy
IF：13.493
Article：MicroRNA-3613-5p Promotes Lung Adenocarcinoma Cell Proliferation through a RELA and AKT/MAPK Positive Feedback Loop
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.889
Article：miR-4721, Induced by EBV-miR-BART22, Targets GSK3b to Enhance the Tumorigenic Capacity of NPC through the WNT/b-catenin Pathway
Periodicals：Molecular Therapy: Nucleic Acids
IF：8.886
Article：MicroRNA-4476 promotes glioma progression through a miR-4476/APC/β-catenin/c-Jun positive feedback loop
Periodicals：Cell Death & Disease
IF：8.469
Article：MiR-4310 induced by SP1 targets PTEN to promote glioma progression
Periodicals：Cancer Cell International
IF：4.178
【In 2019】
Article：Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression
Periodicals：NATURE COMMUNICATIONS
IF：12.123
Article：Timeless-Stimulated miR-5188-FOXO1/b-Cateninc-Jun Feedback Loop Promotes Stemness via Ubiquitination of b-Catenin in Breast Cancer
Periodicals：Molecular Therapy
IF：11.455
Article：HBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma
Periodicals：Theranostics
IF：8.698
Article：VPS33B interacts with NESG1 to modulate EGFR/PI3K/AKT/c-Myc/P53/miR-133a-3p signaling and induce 5-fluorouracil sensitivity in nasopharyngeal carcinoma
Periodicals：Cell Death & Disease
IF：8.469
Article：Cinobufotalin powerfully reversed EBV-miR-BART22-induced cisplatin resistance via stimulating MAP2K4 to antagonize non-muscle myosin heavy chain IIA/glycogen synthase 3β/β-catenin signaling pathway
Periodicals：EBioMedicine
IF：5.736
Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant
Periodicals：Cellular and Molecular Neurobiology
IF：3.606
【In 2016】
Article：miR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTOR–p-PI3K/AKT-c-JUN
Periodicals：NATURE COMMUNICATIONS
IF：14.92
