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GST pulldown Kit

The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system.

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PRODUCT INFORMATION

Product introduction：

The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system.The target protein-GST fusion protein is affinity immobilized on the glutathione affinity resin, which acts as a support for affinity with the target protein and acts as a "bait protein".The target protein solution passes through the column to capture the "capture protein (target protein)" that interacts with it.The eluted conjugate was separated by SDS-PAGE electrophoresis, and finally subjected to Western Blot to confirm the interaction between the two proteins.Both "bait protein" and "capture protein" can be obtained by methods such as cell lysates, purified proteins, expression systems, and in vitro transcription and translation systems.(This kit is for scientific research purposes only)

Technical process：

Product features：

1.BersinbioTM's GST pulldown Kit can ensure that the GST tag fusion protein is not degraded.
2.Provide required reagents and detailed operating instructions, including protein purification process.
3.The ordinary equipment in the laboratory can be operated, and the process is simple.
4.Simple operation, short time-consuming, objective and accurate results.

5.The first and exclusive product in China with independent intellectual property rights.

Instructions

Bes3012 GST-pulldown

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