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CLIP-qPCR Kit

CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation.

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PRODUCT INFORMATION

Product introduction：

CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation.CLIP-qPCR obtains RNA after digestion (Protease K, DNase I, RNase T1) by immunoprecipitation of the binding complex of RBP and RNA.By adding a linker to the 3'end of the RNA and designing primers for different sites, the enrichment efficiency of RNA-binding protein sites can be detected.(This kit is for scientific research purposes only)

Technical process：

Product features：

1.After ultraviolet irradiation, the RNA in the cell is cross-linked with the corresponding RNA-binding protein to enhance the binding ability of RNA and protein.
2.Can detect a variety of RNA, such as lncRNA, mRNA, microRNA.
3.CLIP-qPCR can accurately locate the binding site of RNA and protein.

4.Have independent intellectual property rights.

Instructions

Bes3014-1 CLIP-qPCR

Bes3014-2 CLIP-seq

Articles

【In 2023】

Article：The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma

Periodicals：The Journal of Clinical Investigation

IF：15.900

Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

Periodicals：Translational Research

IF：10.171