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Luciferase reporter gene vector

The Luciferase reporter gene system is a reporter system that uses Luciferin as a substrate to detect the activity of Firefly Luciferase.
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Product introduction:
The Luciferase reporter gene system is a reporter system that uses Luciferin as a substrate to detect the activity of Firefly Luciferase.The traditional application areas of this technology mainly include:
1.Detection of promoter activity.
2.Detect the interaction between transcription factors and target promoters.
3.Detect the regulatory effect of miRNA on target genes.
Reporter gene detection is an important tool in the field of modern molecular biology research to analyze the interaction between potential cis-elements (such as promoters, enhancers, and silencers) and trans-acting factors in the flanking regions of structural genes.Luciferase catalyzes the oxidation of luciferin to Oxyluciferin with the participation of Mg2+ and O2. During the oxidation of luciferin, it emits Bioluminomescence. Then the biofluorescence is measured by a chemiluminescence meter.(For scientific research purposes only)