The hapten is located by immunohistochemistry, which indirectly shows the hybrid formed by the probe and the target nucleic acid, so as to perform qualitative, semi-quantitative and relative positioning analysis of the target nucleic acid.
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Product introduction:
BersinbioTM has established an ISH Probe preparation platform to undertake customized ISH Kit business, which can be customized according to customer needs.
This kit is mainly used to detect the expression level and expression position of the target gene in the sample, and can be used for the detection of tissue sections (paraffin section/frozen section),
Round coverslip and chromosome section. (This kit is for scientific research purposes only)
BersinbioTM CISH Kit series has been welcomed and affirmed by the majority of scientific research users since its listing, and has provided high-quality services to nearly a thousand users including domestic first-class scientific research institutes, medical institutions and multinational biological enterprises. For example, Chinese Academy of Sciences, Sun Yat-sen University School of Medicine and Affiliated Hospital, Southern Medical University School of Medicine and Affiliated Hospital, University of Hong Kong, Peking University School of Medicine and Affiliated Hospital, Southeast University School of Medicine, Jiangxi Agricultural University, Tongji Medical College of Huazhong University of Science and Technology, etc.
At the same time, after years of accumulation, BersinbioTM has a strong in situ hybridization technology team to provide technical support and answers to various problems encountered by customers in the use of the kit.
At present, BersinbioTM has nearly a thousand kinds of stock kits: among them, there are about 1,100 kinds of mRNA; about 500 kinds of microRNA; species include humans, rats, mice, rabbits, pigs, chickens, bacteria, insects, and so on.

Principle of the experiment:
The target nucleic acid on the cell or tissue section to be detected is homologous and complementary to the nucleic acid probe used, and the two are denatured, annealed, and renatured to form a hybrid of the target nucleic acid and the nucleic acid probe. A certain nucleotide of the nucleic acid probe is labeled with the hapten DIG. Finally, the hapten is located by immunohistochemistry, which indirectly shows the hybrid formed by the probe and the target nucleic acid, so as to perform qualitative, semi-quantitative and relative positioning analysis of the target nucleic acid.

Technical process:

Result example:

Product features:

1、Safe, fast, and reproducible.
2、The specificity is 100% and the sensitivity exceeds 90%.
3、The probe has stable performance and can be stored at low temperature for more than two year.
4、Simple operation, precise positioning, no experimental pollution.

5、Customize personalized probes according to customer needs.


Bes1006 CISH(short chain)

Bes1007 CISH(long chain)


【In 2023

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ArticleLong non-coding RNA FENDRR suppresses cancer-associated fibroblasts and serves as a prognostic indicator in colorectal cancer

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ArticleRole of LncRNA MIR99AHG in breast cancer: Bioinformatic analysis and preliminary verification



【In 2022

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Article:Effects of miR-939 and miR-376A on ulcerative colitis using a decoy strategy to inhibit NF-κB and NFAT expression

Periodicals:European Journal of Histochemistry


【In 2020

ArticleAn NF-κBdriven lncRNA orchestrates colitis and circadian clock



ArticleMiR-4310 induced by SP1 targets PTEN to promote glioma progression

PeriodicalsCancer Cell International


ArticlelncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR106a5pmediated P38 MAPK signaling

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【In 2019

ArticleLnc-TALC promotes O6-methylguanine-DNA methyltransferase expression via regulating the c-Met pathway by competitively binding with miR-20b-3p



ArticleHBX-induced miR-5188 impairs FOXO1 to stimulate β-catenin nuclear translocation and promotes tumor stemness in hepatocellular carcinoma



Article:Timeless-Stimulated miR-5188-FOXO1/b-Cateninc-Jun Feedback Loop Promotes Stemness via Ubiquitination of b-Catenin in Breast Cancer

Periodicals:Molecular Therapy


ArticleThe long noncoding RNA AK002107 negatively modulates miR-140-5p and  targets TGFBR1 to induce epithelial-mesenchymal transition in hepatocellular carcinoma

PeriodicalsMolecular Oncology


ArticleCancer-associated fibroblasts promote PD-L1 expression in mice cancer cells via secreting CXCL5

PeriodicalsInternational Journal of Cancer


【In 2018

ArticleRETRACTED ARTICLE: Dual roles of miR-374a by modulated c-Jun respectively targets CCND1-inducing PI3K/AKT signal and PTEN-suppressing Wnt/β-catenin signaling in non-small-cell lung cancer

PeriodicalsCell Death & Disease


ArticleInhibition of microRNA-16 facilitates the paclitaxel resistance by targeting IKBKB via NF-kB signaling pathway in hepatocellular carcinoma

PeriodicalsBiochemical and Biophysical Research Communications


【In 2017

ArticleOCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation

PeriodicalsBioMed Research International


【In 2016

ArticlemiR-3188 regulates nasopharyngeal carcinoma proliferation and chemosensitivity through a FOXO1-modulated positive feedback loop with mTORp-PI3K/AKT-c-JUN



ArticleLong non-coding RNA LOC389641 promotes progression of pancreatic ductal adenocarcinoma and increases cell invasion by regulating E-cadherin in a TNFRSF10A-related manner

PeriodicalsCancer Letters