After denaturation, annealing, renaturation, and washing, a hybrid of target DNA and nucleic acid probe is formed. Finally, it is developed under a fluorescence microscope to perform qualitative and localization analysis of the DNA to be tested.
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Product introduction:
BersinbioTM has established a FISH Probe preparation platform to undertake customized fluorescence in situ hybridization kit business, which can be customized according to customer needs.
This kit is mainly used to detect the expression level and expression position of the target gene in the sample, and can be used for the detection of tissue sections (paraffin sections/frozen sections), Round coverslip and chromosome section.(This kit is for scientific research purposes only)

BersinbioTM FISH Kit series has been welcomed and affirmed by the majority of scientific research users since its listing, and has provided high-quality services to nearly a thousand users including domestic first-class scientific research institutes, medical institutions and multinational biological enterprises. For example, Chinese Academy of Sciences, Sun Yat-sen University School of Medicine and Affiliated Hospital, Southern Medical University School of Medicine and Affiliated Hospital, University of Hong Kong, Peking University School of Medicine and Affiliated Hospital, Southeast University School of Medicine, Jiangxi Agricultural University, Tongji Medical College of Huazhong University of Science and Technology, etc.
BersinbioTM uses the self-obtained specific probes from the research species and chemically covalently connects them with fluorescein. Autofluorescence probes have obvious advantages: high sensitivity, good light stability, and long fluorescence lifetime; the biggest advantage is that autofluorescence probes can effectively reduce self-specific fluorescence interference and achieve more efficient and specific gene detection signals. The comparison will produce great technical effects, gain customer approval and replace traditional probe detection, and meet the experimental needs of domestic and foreign scientific researchers for high-quality FISH Kits.
The research and development of the kit has received strong financial and policy support from the National Science and Technology Enterprise Innovation Fund and the Guangdong Province and Guangzhou Municipal Science and Technology Bureaus at all levels. The core technology of this product has applied for a national patent, which will protect the core technology and related supporting indicators. At the same time, after years of accumulation, a molecular probe research and development center was established in 2011. BersinbioTM has a strong fluorescence in situ hybridization technology team to provide technical support and answers to various problems encountered by customers in the use of the kit.

Principle of the experiment:
FISH is to hybridize the fluorescein-labeled oligonucleotide probe with the nucleic acid of the denatured chromosome according to the principle of base complementary pairing. After denaturation, annealing, renaturation, and washing, a hybrid of target DNA and nucleic acid probe is formed. Finally, it is developed under a fluorescence microscope to perform qualitative and localization analysis of the DNA to be tested.

Technical process:

Result example:

Product features:

1、Safe, fast, and reproducible.
2、The specificity is 100% and the sensitivity exceeds 90%.
3、The probe is a direct labeling method, eliminating the need for a signal amplification system.
4、The probe has stable performance and can be stored at low temperature for more than one year.
5、The fluorescence signal is clear, and the result judgment is intuitive and reliable.
6、Simple operation, precise positioning, no experimental pollution.
7、Customize personalized probes according to customer needs.


Bes1001 RNA-FISH(short chain)

Bes1002 RNA-FISH(long chain) 

Bes1011 DNA-FISH


【In 2023

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ArticleCircFOXK2 promotes hepatocellular carcinoma progression and  leads to a poor clinical prognosis via regulating the Warburg efect

PeriodicalsJournal of Experimental &Clinical Cancer Research


ArticleMicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer

PeriodicalsCell Death and Disease


ArticleMarsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1-dependent inhibition of Wnt/β-catenin pathway

Periodicals:CNS Neuroscience & Therapeutics


ArticleCircular RNA hsa_circ_0005218 promotes the early recurrence of hepatocellular carcinoma by targeting the miR-31-5p/CDK1 pathway



ArticleSLERT, as a novel biomarker, orchestrates endometrial cancer metastasis via regulation of BDNF/TRKB signaling

PeriodicalsWorld Journal of Surgical Oncology


ArticleScreening and Bioinformatics Analyses of Key miRNAs Associated with Toll-like Receptor Activation in Gastric Cancer Cells



【In 2022

ArticleTLR4 regulates RORγt+ regulatory T-cell responses and  susceptibility to colon infammation through interaction with Akkermansia muciniphila



ArticleRole of lncSLCO1C1 in gastric cancer progression and resistance to oxaliplatin therapy

PeriodicalsClinical and Translational Medicine


ArticlecircCYP24A1 promotes Docetaxel resistance in prostate Cancer by Upregulating ALDH1A3

PeriodicalsBiomarker Research


ArticleSerum-derived extracellular vesicles facilitate temozolomide resistance in glioblastoma through a HOTAIR-dependent mechanism

PeriodicalsCell Death & Disease


ArticleSIX5-activated LINC01468 promotes lung adenocarcinoma progression by recruiting SERBP1 to regulate SERPINE1 mRNA stability and recruiting USP5 to facilitate PAI1 protein deubiquitylation

PeriodicalsCell Death & Disease


ArticleBone marrow mesenchymal stem cells inhibit hepatic fbrosis via the AABR07028795.2/rno-miR-667-5p axis

PeriodicalsStem Cell Research & Therapy


ArticleCircular RNA circLAMA3 inhibits the proliferation of bladder cancer by directly binding an mRNA

PeriodicalsMolecular Therapy: Oncolytics


ArticleCircular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression

PeriodicalsCell Proliferation


ArticleOLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fbroblasts to participate in Randalls plaque formation

PeriodicalsMolecular Medicine


ArticlecircDNMT1 Promotes Malignant Progression of Gastric Cancer Through Targeting miR-576-3p/Hypoxia Inducible Factor-1 Alpha Axis

PeriodicalsFrontiers in Oncology


ArticleLong noncoding RNA CASC7 is a novel regulator of glycolysis in oesophageal cancer via a miR-143-3p-mediated HK2 signalling pathway

PeriodicalsCell Death Discovery


ArticleLong non-coding RNA lncC11orf54-1 modulates neuroinfammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection

PeriodicalsMolecular Brain


ArticleMiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway

PeriodicalsRenal Failure


【In 2021

ArticleATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking

Periodicalsnature communications


ArticleLong noncoding RNA IL6-AS1 is highly expressed in chronic obstructive pulmonary disease and is associated with interleukin 6 by targeting miR-149-5p and early B-cell factor 1

PeriodicalsClinical and translational medicine


ArticleCircRNA Chordc1 protects mice from abdominal aortic aneurysm by contributing to the phenotype and growth of vascular smooth muscle cells

PeriodicalsMolecular Therapy: Nucleic Acids


ArticleOscillating lncRNA Platr4 regulates NLRP3 inflammasome to ameliorate nonalcoholic steatohepatitis in mice



ArticlecircATP2B1 Promotes Aerobic Glycolysis in Gastric Cancer Cells Through Regulation of the miR-326 Gene Cluster

PeriodicalsFrontiers in Oncology


ArticleGlobal identification of fulllength cassava lncRNAs unveils the role of coldresponsive intergenic lncRNA 1 in cold stress response

PeriodicalsPlant, Cell & Environment


ArticleHsa_circ_0000652 Aggravates Inflammation by Activation of Macrophages and Enhancement of OX40/OX40L Interaction in Ankylosing Spondylitis

PeriodicalsFrontiers in Cell and Developmental Biology


ArticleIdentification of low-dose radiation-induced exosomal circMETRN and miR-4709-3p/GRB14/PDGFRα pathway as a key regulatory mechanism in Glioblastoma progression and radioresistance: Functional validation and clinical theranostic significance

PeriodicalsInternational Journal of Biological Sciences


ArticleLncRNA Nqo1-AS1 Attenuates Cigarette Smoke-Induced Oxidative Stress by Upregulating its Natural Antisense Transcript Nqo1

PeriodicalsFrontiers in Pharmacology


ArticleHsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction



ArticlePaeonol inhibits the progression of intracerebral haemorrhage by mediating the HOTAIR/UPF1/ACSL4 axis

PeriodicalsASN Neuro


ArticleTumor-derived exosomal circRNA051239 promotes proliferation and migration of epithelial ovarian cancer

PeriodicalsAm J Transl Res


ArticleIdentification and Functional Prediction of Long Non-Coding RNAs in Dilated Cardiomyopathy by Bioinformatics Analysis

PeriodicalsFrontiers in Genetics


ArticleRole of long intergenic non-protein coding RNA 01857 in hepatocellular carcinoma malignancy via the regulation of the microRNA-197-3p/anterior GRadient 2 axis

PeriodicalsPLOS ONE


ArticleLong noncoding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA588 in colorectal cancer

PeriodicalsOncology Letters


ArticleMicroRNA-488-3p Regulates Neuronal Cell Death in Cerebral Ischemic Stroke Through Vacuolar Protein Sorting 4B (VPS4B)

PeriodicalsNeuropsychiatric Disease and Treatment


ArticlelncRNA RP11531A24.3 inhibits the migration and proliferation of vascular smooth muscle cells by downregulating ANXA2 expression

PeriodicalsExperimental and Therapeutic Medicine


ArticleLong noncoding RNA SNHG8 promotes autophagy as a ceRNA to upregulate ATG7 by sponging microRNA588 in colorectal cancer

PeriodicalsOncology Letters


Development history:
In 2011, the FISH Probe preparation platform was established to undertake customized fluorescence in situ hybridization kit business, which can be customized according to customer needs.
In 2012, the FISH Kit was successfully developed, the kit production line was established, and the product was put on the market.
In 2013, the Chinese and English registration of the BersinbioTM trademark was completed. In the same year, the BersinbioTM FISH Kit core technology invention patent was recognized and approved by the State Intellectual Property Office. The patent will protect the core technology and related supporting indicators. The BersinbioTM FISH Kit has been recognized by customers.
In 2014, BersinbioTM passed the ISO 9001 quality management system certification.

Cytogenetics Research:
Chromosome number and chromosome segment deletion, translocation, inversion, duplication and other chromosomal structural variation detection.
Gene mapping and map construction:
Directly and quickly determine the relationship between the target DNA sequence and telomeres, chromosome bands and centromeres, for use in the construction of genetic physical maps.
Transgenic cytological identification:
Design probes with the target gene as the target sequence, locate the position of the transformed gene on the chromosome, and detect whether the transgene exists in the nucleus of the recipient cell and whether it is integrated into the chromosome.
Genome evolution research:
Using the total DNA of the hybrid parent as the label probe, and the genomic DNA of the other parent as the blocking DNA, in situ hybridization is performed on the chromosome preparation of the hybrid to effectively distinguish two chromosomes with similar homology.
Gene expression research:
It is applied to the expression and location analysis of mRNA, miRNA and lncRNA.
Microbiological testing research:
Oligonucleotide probes are designed with species-specific nucleotide sequences such as 16S rRNA, 23S rRNA and their spacers as targets, which are mainly used in microbial system development, microbial diagnosis and environmental microbial ecology research.
Possess more than 10,000 kinds of conventional genes and more than 1,000 kinds of microRNA.