Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats
research target：rat
Periodicals：Biochemical and Biophysical Research Communications(BBIC)
IF：2.484
Cooperative Unit：Pearl River Hospital, Southern Medical University
Time of publication：September 21, 2015
Summary
Background: Adipose-derived stem cells (ADSCs) were effective in treating wound. Stromal cell-derived factor-1 (SDF-1), a chemokine usually called CXCL12, is well known for its chemotaxis in induction of cell migration. However, little is known about the SDF-1responsible for the complex migration of ADSCs from residence to injured sites.
Objective: Herein, we firstly showed SDF-1 is a major regulator involved in migration of ADSCs during wound repair in vivo.
Methods: Trauma in rats was induced by surgical operation. The levels of SDF-1 in wounded tissue were assayed by ELISA. ADSCs were labeled with Green Fluorescent Protein (GFP), and then were transferred to injured rats by intracarotid injection. The plasma levels of ADSCs during wound healing were detected by flow cytometry, and ADSCs in injured tissue were evaluated by bioluminescence imaging in vivo and laser confocal microscopy (LCM), respectively.
Results: ADSCs were successfully labeled with GFP. SDF-1 level reached to the peak value on 24 h after injury and then decreased continuously. Additionally, levels of plasma ADSCs in SDF-1 treated rats reached to the peak value (12%) at d21 after medicine delivery, while those of normal and injured rats showed the peak values of 6.28% and 9.84% at d7 and d21, respectively. Finally, the results of LCM indicated treatment of ectogenic SDF-1 obviously enhanced GFP-ADSCs distribution in wounded tissues.
Conclusion: Our results indicated that SDF-1 treatment obviously promoted the migration and directed distribution of ADSCs in traumatic tissue.
1. Using lentivirus transfection, GFP successfully labeled adipose stem cells (ADSCs), and flow cytometry detected GFP successfully labeled adipose stem cells (ADSCs) reached more than 80%.
2. The migration of adipose-derived stem cells (ADSCs) in exogenous SDF-1-treated plasma was enhanced.The level of adipose stem cells (ADSCs) in plasma detected by flow cytometry at different time points reached a peak (12%) at 21d, which was significantly higher than that of the normal and untreated groups.The results showed that SDF-1 treatment significantly enhanced the migration of adipose stem cells (ADSCs).
3. Exogenous SDF-1 enhances migration of adipose stem cells (ADSCs) in the wounded area.Bioluminescence imaging was used to detect GFP-ADSCs in wounded tissues, and the results at different treatment time points showed that the migration of GFP-ADSCs in wounded tissues was enhanced in the presence of SDF-1.
4. Enrichment of exogenous SDF-1 for the distribution of adipose stem cells (ADSCs) in the wound area.The wound tissue at different time points after experimental treatment was frozen sectioned and observed by confocal microscope. Compared with the PBS-treated group, the number of adipose stem cells (ADSCs) in the SDF-1-treated group showed a significant increase.The results showed that SDF-1 enhanced the migration of adipose stem cells (ADSCs) to accelerate wound repair.
References
Wu Q, Ji F K, Wang J H, et al. Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats.[J]. Biochemical & Biophysical Research Communications, 2015, 467(1):140-5.
