Product introduction：
The interaction between protein and RNA is the core of many cell functions, such as protein synthesis, mRNA assembly, virus replication, cell development regulation, etc. Understanding the molecular mechanism of their interaction is very important for understanding these biological processes.RNA pulldown technology utilizes desulfobiotin-labeled RNA and streptavidin-labeled magnetic beads to efficiently enrich and identify RBPs.
A specific RNA probe labeled with desthiobiotin is designed for the target area and incubated with cell protein extracts.The target RNA probe-protein complex is obtained by coupling magnetic beads and eluting.Finally, Western Blot or MS was used to identify protein types.(This kit is for scientific research purposes only)
Technical process：
Product features：
1.High quality, good stability.
2.Simple operation and easy to use.
3.High specificity and low background.
4.Have independent intellectual property patents.
5.Fast and time-saving: all experiments only need 3 hours.
Instructions
Bes5102 RNA pulldown（12T、24T、40T）
Articles
【In 2023】
Article：CircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications
Periodicals：Nature Communications
IF：16.600
Article：Integrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma
Periodicals：Cancer Research
IF：11.200
Article：Upregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis
Periodicals：Clinical and Translational Medicine
IF：10.600
Article：Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration
Periodicals：Clinical and Translational Medicine
IF：10.600
Article：CircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling
Periodicals：Translational Research
IF：10.17
Article：Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis
Periodicals：BMC Medicine
IF：9.300
Article： Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis
Periodicals：International Journal of Biological Sciences
IF：9.200
Article：Nucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription
Periodicals：Cancer Science
IF：5.700
Article：Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes
Periodicals：International Journal of Molecular Sciences
IF：5.600
Article：SNHG25 promotes colorectal cancer metastasis by regulating MMP2.
Periodicals：Aging (Albany NY)
IF：5.200
Article：F-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner
Periodicals：Cancers
IF：5.200
Article：RBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer
Periodicals：International Journal of Oncology
IF：5.200
Article：FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas
Periodicals：Heliyon
IF：4.000
Article：LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter
Periodicals：BMC Cancer
IF：3.800
Article： IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway.
Periodicals：Epigenetics
IF：3.700
【In 2022】
Article：Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：11.300
Article：PHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer
Periodicals：International Journal of Biological Sciences
IF：9.200
Article：LINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2
Periodicals：Cell Death and Disease
IF：9.865
Article：A reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic infammatory microenvironment
Periodicals：Stem Cell Research & Therapy
IF：6.832
Article：LncRNA USP2-AS1 Promotes Hepatocellular Carcinoma Growth by Enhancing YBX1-Mediated HIF1a Protein Translation Under Hypoxia
Periodicals：Frontiers in Oncology
IF：6.243
Article：LINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia
Periodicals：Scientifc Reports
IF：4.996
【In 2021】
Article：Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：11.16
Article：Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress
Periodicals：Theranostics
IF：8.579
Article：LINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer
Periodicals：Cell Death & Disease
IF：8.469
Article：LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma
Periodicals：Cell Death & Disease
IF：8.469
Article：LINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2
Periodicals：Clinical and Translational Medicine
IF：7.915
Article：CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs
Periodicals：Cell Death & Disease
IF：6.304
【In 2020】
Article：Gut microbiota regulate tumor metastasis via circRNA/miRNA networks
Periodicals：Gut Microbes
IF：7.74
Article：Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways
Periodicals：Environment International
IF：7.577
【In 2019】
Article：Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family
Periodicals：Advanced Science
IF：17.521
Article：Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer
Periodicals：Molecular Cancer
IF：7.776
【In 2018】
Article：Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN
Periodicals：The Journal of Clinical Investigation
IF：13.251
Article：Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis
Periodicals：Society of Toxicology
IF：4.181
References:
[1]Shen S,Wu Y,Chen J,et al.CircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene[J].Annals of the rheumatic diseases,2019,78(6):826-836.DOI:10.1136/annrheumdis-2018-214786
Original link:https://ard.bmj.com/content/78/6/826
[2]Nan A,Jia Y,Li X,et al.Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis[J].Toxicological sciences : an official journal of the Society of Toxicology,2018,161(1):115-124.DOI:10.1093/toxsci/kfx203
Original link:https://academic.oup.com/toxsci/article/161/1/115/4211135?searchresult=1
[3]Chen L,Nan A,Zhang N,et al.Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer[J].Molecular cancer,2019,18(1):13.DOI:10.1186/s12943-019-0943-0
Original link:https://doi.org/10.1186/s12943-019-0943-0
