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RNA pulldown Kit

A specific RNA probe labeled with desthiobiotin is designed for the target area and incubated with cell protein extracts.The target RNA probe-protein complex is obtained by coupling magnetic beads and eluting.
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PRODUCT INFORMATION

Product introduction:
The interaction between protein and RNA is the core of many cell functions, such as protein synthesis, mRNA assembly, virus replication, cell development regulation, etc. Understanding the molecular mechanism of their interaction is very important for understanding these biological processes.RNA pulldown technology utilizes desulfobiotin-labeled RNA and streptavidin-labeled magnetic beads to efficiently enrich and identify RBPs.
A specific RNA probe labeled with desthiobiotin is designed for the target area and incubated with cell protein extracts.The target RNA probe-protein complex is obtained by coupling magnetic beads and eluting.Finally, Western Blot or MS was used to identify protein types.(This kit is for scientific research purposes only)


 





Technical process:







Product features:


1.High quality, good stability.
2.Simple operation and easy to use.
3.High specificity and low background.
4.Have independent intellectual property patents.
5.Fast and time-saving: all experiments only need 3 hours.


Instructions


Bes5102 RNA pulldown


Articles

【In 2023


ArticleCircRREB1 mediates lipid metabolism related senescent phenotypes in chondrocytes through FASN post-translational modifications

Periodicals:Nature Communications

IF16.600


ArticleIntegrative Single-Cell Analysis Reveals Transcriptional and Epigenetic Regulatory Features of Clear Cell Renal Cell Carcinoma

PeriodicalsCancer Research

IF11.200


ArticleUpregulation of LINC00501 by H3K27 acetylation facilitates gastric cancer metastasis through activating epithelial-mesenchymal transition and angiogenesis

Periodicals:Clinical and Translational Medicine

IF:10.600


ArticleCircular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration

PeriodicalsClinical and Translational Medicine

IF10.600


ArticleCircFOXP1 alleviates brain injury after acute ischemic stroke by regulating STAT3/apoptotic signaling

PeriodicalsTranslational Research

IF10.17


ArticleDownregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis

PeriodicalsBMC Medicine

IF:9.300


Article Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis

Periodicals:International Journal of Biological Sciences

IF9.200


ArticleNucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription

Periodicals:Cancer Science

IF:5.700


Article:Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes

Periodicals:International Journal of Molecular Sciences
IF:5.600


Article:SNHG25 promotes colorectal cancer metastasis by regulating MMP2.

Periodicals:Aging (Albany NY)
IF:5.200


ArticleF-Box Protein 43, Stabilized by N6-Methyladenosine Methylation, Enhances Hepatocellular Carcinoma Cell Growth and Invasion via Promoting p53 Degradation in a Ubiquitin Conjugating Enzyme E2 C-Dependent Manner

PeriodicalsCancers

IF:5.200


ArticleRBM15‑mediating MDR1 mRNA m6A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer

Periodicals:International Journal of Oncology

IF:5.200


ArticleFLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

Periodicals:Heliyon

IF:4.000


ArticleLINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter

PeriodicalsBMC Cancer

IF:3.800


Article IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway.

PeriodicalsEpigenetics

IF:3.700


【In 2022


ArticleLong non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and  lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF11.300


ArticlePHGDH Inhibits Ferroptosis and Promotes Malignant Progression by Upregulating SLC7A11 in Bladder Cancer

PeriodicalsInternational Journal of Biological Sciences

IF:9.200


ArticleLINC00924-induced fatty acid metabolic reprogramming facilitates gastric cancer peritoneal metastasis via hnRNPC-regulated alternative splicing of Mnk2

PeriodicalsCell Death and Disease

IF9.865


ArticleA reduced level of the long non-coding RNA SNHG8 activates the NF-kappaB pathway by releasing functional HIF-1alpha in a hypoxic infammatory microenvironment

PeriodicalsStem Cell Research & Therapy

IF6.832


ArticleLncRNA USP2-AS1 Promotes Hepatocellular Carcinoma Growth by Enhancing YBX1-Mediated HIF1a Protein Translation Under Hypoxia

PeriodicalsFrontiers in Oncology

IF6.243


ArticleLINC00839 promotes malignancy of liver cancer via binding FMNL2 under hypoxia

PeriodicalsScientifc Reports

IF4.996


【In 2021


ArticleSuper-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc

PeriodicalsJournal of Experimental & Clinical Cancer Research

IF11.16


Article:Novel role of circRSU1 in the progression of osteoarthritis by adjusting oxidative stress

Periodicals:Theranostics

IF:8.579


ArticleLINC-PINT impedes DNA repair and enhances radiotherapeutic response by targeting DNA-PKcs in nasopharyngeal cancer

PeriodicalsCell Death & Disease

IF8.469


Article:LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma

Periodicals:Cell Death & Disease

IF:8.469


ArticleLINC01133 promotes hepatocellular carcinoma progression by sponging miR-199a-5p and activating annexin A2

PeriodicalsClinical and Translational Medicine

IF7.915


ArticleCircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and  interacting with microRNAs

PeriodicalsCell Death & Disease

IF6.304


【In 2020


ArticleGut microbiota regulate tumor metastasis via circRNA/miRNA networks

PeriodicalsGut Microbes

IF7.74


Article:Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways

Periodicals:Environment International

IF:7.577


【In 2019


Article:Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family

PeriodicalsAdvanced Science

IF17.521


ArticleCircular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

PeriodicalsMolecular Cancer

IF7.776


【In 2018


ArticleLong noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN

PeriodicalsThe Journal of Clinical Investigation

IF13.251


ArticleEditor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis

PeriodicalsSociety of Toxicology

IF4.181



References:
[1]Shen S,Wu Y,Chen J,et al.CircSERPINE2 protects against osteoarthritis by targeting miR-1271 and ETS-related gene[J].Annals of the rheumatic diseases,2019,78(6):826-836.DOI:10.1136/annrheumdis-2018-214786
Original link:https://ard.bmj.com/content/78/6/826
[2]Nan A,Jia Y,Li X,et al.Editor's Highlight: lncRNAL20992 Regulates Apoptotic Proteins to Promote Lead-Induced Neuronal Apoptosis[J].Toxicological sciences : an official journal of the Society of Toxicology,2018,161(1):115-124.DOI:10.1093/toxsci/kfx203
Original link:https://academic.oup.com/toxsci/article/161/1/115/4211135?searchresult=1
[3]Chen L,Nan A,Zhang N,et al.Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer[J].Molecular cancer,2019,18(1):13.DOI:10.1186/s12943-019-0943-0
Original link:https://doi.org/10.1186/s12943-019-0943-0