Product introduction：
DNA pulldown is a technique used to study the interaction between DNA and protein.The DNA pulldown Kit designs specific DNA probes for the target region, and the probes are labeled with desulphur biotin.Streptavidin is coupled to the magnetic beads and binds to desulphur biotin affinity.The cell nuclear extract is incubated with magnetic beads-DNA probe, and the protein molecule can specifically bind with the DNA probe.After washing, non-specific binding protein molecules can be removed.Finally, elution is performed in the eluent (for streptavidin) to obtain the target DNA probe-protein complex, and then the protein type is identified by Western blot or MS.(This kit is for scientific research purposes only)
Technical process：
Product features：
1.Fast and time-saving: From labeling probes to result analysis, all experiments only take 3 hours.
2.Stable and reliable: DNA ends are labeled with biotin, which does not affect the protein binding site. The labeled DNA can be stored for one year.
3.Completely equipped: The kit includes a control system, and you only need to prepare your own biotin-end-labeled DNA probes and nuclear protein samples.
4.Can obtain complete, high-concentration protein for mass spectrometry detection.
Instructions
Bes5004 DNA pulldown
Articles
【In 2022】
Article：TCF7L2 promotes anoikis resistance and metastasis of gastric cancer by transcriptionally activating PLAUR
Periodicals：International Journal of Biological Sciences
IF：9.200
Article：GLIS2 Prevents Hepatic Fibrosis by Competitively Binding HDAC3 to Inhibit Hepatic Stellate Cell Activation
Periodicals：Cellular and Molecular Gastroenterology and Hepatology
IF：7.200
Article：Insight into mechanisms of pig lncRNA FUT3-AS1 regulating E. coli F18-bacterial diarrhea
Periodicals：PLOS PATHOGENS
IF：6.700
【In 2020】
Article：Spo0A can efficiently enhance the expression of the alkaline protease gene aprE in Bacillus licheniformis by specifically binding to its regulatory region
Periodicals：International Journal of Biological Macromolecules
IF：8.200
Article：VviWRKY40, a WRKY Transcription Factor, Regulates Glycosylated Monoterpenoid Production by VviGT14 in Grape Berry
Periodicals：Genes (Basel)
IF：3.500
【In 2019】
Article：An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry (Morus notabilis)
Periodicals：International Journal of Molecular Sciences
IF：5.600
