Product introduction：
ChIP is an ideal technology for studying the interaction between proteins and DNA in the body. It is currently the best method to determine the genomic region that binds to a specific protein, or to determine the protein that binds to a specific genomic region.ChIP combined with other experimental techniques can be used for detection of different purposes.The ChIP-Seq method is to purify and construct a library of specifically enriched DNA fragments bound by the target protein, and then perform high-throughput sequencing on the enriched DNA fragments.(This kit is for scientific research purposes only)
In the state of living cells, the protein and DNA in the cell are cross-linked, and ultrasound cuts it randomly into small fragments of chromatin within a certain length.Then immunoprecipitate the protein-DNA complex with the target protein-specific antibody to specifically enrich the DNA fragments bound by the target protein.
Technical process：
Result example：
Product features：
1.Efficient and specific enrichment efficiency; the enrichment ratio of positive and negative controls is >120, and each ChIP reaction only requires 100,000 cells.
2.It has strong compatibility with downstream analysis methods. Compatible downstream analysis methods include: RT-qPCR, gene chip and High-Throughput Sequencing.
3.Excellent technical service team, technical service is efficient, comprehensive and rapid, and can promptly help customers solve the problems encountered in the experiment as soon as possible.
Instructions
Bes5001 ChIP（for animal）
Bes5002 ChIP（for plant）
Article
【In 2023】
Article：Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis
Periodicals：Science Advances
IF：13.600
Article：Low expression of m6A reader YTHDC1 promotes progression of ovarian cancer via PIK3R1/STAT3/GANAB axis
Periodicals：International Journal of Biological Sciences
IF：9.200
Article：Identification of a RAD51B enhancer variant for susceptibility and progression to glioma
Periodicals：Cancer Cell International
IF：5.800
Article：Nucleus-localized circSLC39A5 suppresses hepatocellular carcinoma development by binding to STAT1 to regulate TDG transcription
Periodicals：Cancer Science
IF：5.700
Article：LincRNA00612 inhibits apoptosis and inflammation in LPS-induced BEAS-2B cells via enhancing interaction between p-STAT3 and A2M promoter
Periodicals：PeerJ
IF：2.700
【In 2022】
Article：STAT3-NAV2 axis as a new therapeutic target for rheumatoid arthritis via activating SSH1L/Cofilin-1 signaling pathway
Periodicals：Signal Transduction and Targeted Therapy
IF：39.300
Article：N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer
Periodicals：Nature Communications
IF：16.600
Article：Dual immunological and proliferative regulation of immune checkpoint FGL1 in lung adenocarcinoma: The pivotal role of the YY1–FGL1–MYH9 axis
Periodicals：Frontiers in Immunology
IF：7.300
Article：Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors
Periodicals：PLOS Pathogens
IF：6.700
Article：Sp1 S-Sulfhydration Induced by Hydrogen Sulfide Inhibits Inflammation via HDAC6/MyD88/NF-κB Signaling Pathway in Adjuvant-Induced Arthritis
Periodicals：Antioxidants
IF：6.313
Article：Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development
Periodicals：International Journal of Molecular Sciences
IF：6.208
Article：ARID1A downregulation promotes cell proliferation and migration of colon cancer via VIM activation and CDH1 suppression
Periodicals：Journal of Cellular and Molecular Medicine
IF：5.295
【In 2021】
Article：Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells
Periodicals：Science Advances
IF：13.600
Article：LncRNA H19 governs mitophagy and restores mitochondrial respiration in the heart through Pink1/Parkin signaling during obesity
Periodicals：Cell Death & Disease
IF：9.000
Article：NAV2 positively modulates inflammatory response of fibroblast-like synoviocytes through activating Wnt/β-catenin signaling pathway in rheumatoid arthritis
Periodicals：Clinical and Translational Medicine
IF：7.919
Article：An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma
Periodicals：Cancer Cell International
IF：5.722
【In 2020】
Article：SLC2A3 promotes macrophage infltration by glycolysis reprogramming in gastric cancer
Periodicals：Cancer Cell International
IF：4.175
【In 2019】
Article：Nrf2-regulated miR-380-3p blocks the translation of Sp3 protein and its mediation of paraquat-induced toxicity in mouse neuroblastoma N2a cells
Periodicals：Toxicological Sciences
IF：3.829
Article：Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells
Periodicals：Molecular Biology of the Cell
IF：3.791
Article：Genetic Predisposition to Glioma Mediated by a MAPKAP1 Enhancer Variant
Periodicals：Cellular and Molecular Neurobiology
IF：3.606
【In 2018】
Article：RIF1 promotes human epithelial ovarian cancer growth and progression via activating human telomerase reverse transcriptase expression
Periodicals：Journal of Experimental & Clinical Cancer Research
IF：7.067
【In 2017】
Article：LncRNA PLAC2 down-regulates RPL36 expression and blocks cell cycle progression in glioma through a mechanism involving STAT1
Periodicals：Journal of Cellular and Molecular Medicine
IF：4.302
【In 2015】
Article：Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells
Periodicals：Neuro-Oncology
IF：9.384
