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CUT&Tag Assay Kit

Cleavage Under Target & Tagmentation (CUT&Tag) is an emerging method of studying DNA-protein interaction.
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PRODUCT INFORMATION

Product introduction


Cleavage Under Target & Tagmentation (CUT&Tag) is an emerging method of studying DNA-protein interaction. This kit uses the Tn5 transposase fused to Protein A/G, which binds to antibodies. After the activation of Tn5, the sequences on both sides of the binding site can be connected with the sequencing connector at the same time, and the library can be constructed by PCR amplification after the DNA is recovered from the cell. The components in the kit have undergone strict quality control to ensure product stability and repeatability.

Technical process


In the living cell state, ConA magnetic beads were used to fix the cells, Digitonin was added to penetrate the cell membrane, and the primary antibody, secondary antibody and ProteinA/G fused with the Tn5 transposase was incubated successively, and then Mg2+ was added to activate the transposition enzyme for fragment reaction. The second generation sequencing library was constructed by DNA extraction and PCR amplification of the reaction products.





Product features


1, Easy to operate: the whole process optimization, magnetic bead extraction saving time and safe and efficient;

2, High signal-to-noise ratio: the background is much lower than ChIP-Seq;

3, Low sample input: technical innovation, low cell initiation;

4. Good repeatability: good repeatability between parallel samples;


Instructions


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