Skin Tissue Clearing & Immunofluorescence Kit

Kit Description:

    This kit is suitable for mammalian skin tissues such as human/mouse/rat. It is an immunofluorescence staining kit specifically designed for skin tissues, used for antigen localization and expression analysis of thick tissue samples.

Kit Principle:

    In the process of immunofluorescence staining, this kit specially incorporates tissue clearing treatment. Based on the principles of gradient dehydration, delipidation, and refractive index matching, it achieves tissue clearing. It effectively removes lipids and endogenous pigments in the tissue, unifies the tissue refractive index, reduces light scattering, enables efficient clearing of thick tissues, and retains antigenicity and fluorescent signals. This improves the depth of microscopic imaging and the fluorescence signal-to-noise ratio. The cleared tissues are suitable for three-dimensional imaging using fluorescence microscopes/laser confocal microscopes/spectral microscopes, ensuring intact sample structure and clear signals, which is an important guarantee for high-resolution spatial localization and quantitative analysis.

Kit Applications:

    This product is widely used in the observation of cellular substructures, gene expression research, and pathological mechanism exploration of skin tissues. It is particularly suitable for scientific research experiments requiring high-precision tissue immunofluorescence analysis.

Technical Roadmap:

Instructions:

Bes1026 Skin Tissue Clearing & Immunofluorescence Kit