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According to different research objects, ChIRP technology combines high-throughput sequencing (ChIRP-Seq) and mass spectrometry (ChIRP-MS) to study the genes and proteins that interact with target RNA....
A specific RNA probe labeled with desthiobiotin is designed for the target area and incubated with cell protein extracts.The target RNA probe-protein complex is obtained by coupling magnetic beads and eluting....
RIP is a powerful tool to understand the dynamic process of post-transcriptional regulatory networks. It can be applied to study miRNA regulatory targets, the interaction between RNA and RBPs, etc....
Reverse-ChIP can comprehensively and systematically identify proteins related to target DNA sites, especially looking for regulatory proteins corresponding to known DNA elements....
MeDIP enriches highly methylated DNA fragments with 5mC antibody, and uses downstream nucleic acid detection technology to detect methylation sites in the whole genome....
The current adenovirus expression system basically uses the method of homologous recombination to construct the exogenous gene or shRNA expression cassette onto the adenovirus backbone vector....
The use of lentiviral vectors can effectively transfer the target gene or shRNA expression vector into cells or animals...
Co-IP is a classic method for studying protein interactions based on the specificity of binding between antibodies and antigens....
The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system....
CLIP-seq obtains purified and digested (Protease K, DNase I, RNase T1) RNA by immunoprecipitating the binding complex of RBP and RNA, and performs cDNA library construction and sequencing analysis....
Red blood cell lysate uses the principle of rupture of the cell membrane caused by the difference in the concentration of salt ions inside and outside the cell to lyse the non-nucleated red blood cells....
Northern blot is a method of transferring RNA from a gel to a nylon membrane, and detecting gene expression by detecting the level of RNA expression....
CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation....
The Luciferase reporter gene system is a reporter system that uses Luciferin as a substrate to detect the activity of Firefly Luciferase....