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Reverse-ChIP can comprehensively and systematically identify proteins related to target DNA sites, especially looking for regulatory proteins corresponding to known DNA elements....
MeDIP enriches highly methylated DNA fragments with 5mC antibody, and uses downstream nucleic acid detection technology to detect methylation sites in the whole genome....
The current adenovirus expression system basically uses the method of homologous recombination to construct the exogenous gene or shRNA expression cassette onto the adenovirus backbone vector....
The use of lentiviral vectors can effectively transfer the target gene or shRNA expression vector into cells or animals...
Co-IP is a classic method for studying protein interactions based on the specificity of binding between antibodies and antigens....
The GST pulldown experiment is an in vitro test technique that effectively validates the yeast two-hybrid system....
CLIP-seq obtains purified and digested (Protease K, DNase I, RNase T1) RNA by immunoprecipitating the binding complex of RBP and RNA, and performs cDNA library construction and sequencing analysis....
Red blood cell lysate uses the principle of rupture of the cell membrane caused by the difference in the concentration of salt ions inside and outside the cell to lyse the non-nucleated red blood cells....
Northern blot is a method of transferring RNA from a gel to a nylon membrane, and detecting gene expression by detecting the level of RNA expression....
CLIP is an important technology to study the interaction between protein and RNA by using the covalent cross-linking characteristic of protein and RNA under 356 nm ultraviolet light irradiation....
The Luciferase reporter gene system is a reporter system that uses Luciferin as a substrate to detect the activity of Firefly Luciferase....
This product is a DNA Marker labeled with biotin and DIG, which can be used as a DNA Marker to be used in Northern blot experiments to accurately determine the size of the fragments in experiments such as hybridization....
Southern blotting technology can be used in researches such as restriction map analysis of cloned genes, qualitative and quantitative analysis of genes in the genome, gene mutation analysis and restriction gene fragment ......
The protein binds to the DNA fragment, which can protect the binding site from being damaged by DNase, and the fragment (also known as "footprint") is left behind after the DNA molecule is digested....